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91.
92.
Swarbhanu Sarkar Yeong Su Ha Nisarg Soni Gwang Il An Woonghee Lee Min Hwan Kim Phuong Tu Huynh Heesu Ahn Nikunj Bhatt Yong Jin Lee Jung Young Kim Kwon Moo Park Isao Ishii Shin‐Geol Kang Jeongsoo Yoo 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2016,128(32):9511-9516
Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope‐based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin‐layer radiochromatography method. When 64Cu–cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter‐sized infarcted lesion of the rat heart. 相似文献
93.
Separation efficiency of free‐solution conjugated electrophoresis with drag‐tags incorporating a synthetic amino acid 下载免费PDF全文
DNA sequencing or separation by conventional capillary electrophoresis with a polymer matrix has some inherent drawbacks, such as the expense of polymer matrix and limitations in sequencing read length. As DNA fragments have a linear charge‐to‐friction ratio in free solution, DNA fragments cannot be separated by size. However, size‐based separation of DNA is possible in free‐solution conjugate electrophoresis (FSCE) if a “drag‐tag” is attached to DNA fragments because the tag breaks the linear charge‐to‐friction scaling. Although several previous studies have demonstrated the feasibility of DNA separation by free‐solution conjugated electrophoresis, generation of a monodisperse drag‐tag and identification of a strong, site‐specific conjugation method between a DNA fragment and a drag‐tag are challenges that still remain. In this study, we demonstrate an efficient FSCE method by conjugating a biologically synthesized elastin‐like polypeptide (ELP) and green fluorescent protein (GFP) to DNA fragments. In addition, to produce strong and site‐specific conjugation, a methionine residue in drag‐tags is replaced with homopropargylglycine (Hpg), which can be conjugated specifically to a DNA fragment with an azide site. 相似文献
94.
Binh Thanh Nguyen Min Park Jae‐Chul Pyun Young Sook Yoo Min‐Jung Kang 《Electrophoresis》2016,37(23-24):3146-3153
An assay for protein kinase C delta (PKCδ) activity based on the quantification of a synthetic substrate using capillary electrophoresis with laser‐induced fluorescence detection was developed. The peptides labeled with fluorescein isothiocyanate F‐ERK (where ERK is extracellular signal‐regulated kinase) and the phosphorylated form, P‐F‐ERK, were utilized for the method development and validation. The migration time of F‐ERK and P‐F‐ERK were 6.3 ± 0.1 and 8.7 ± 0.2 min, respectively. LOD and LOQ values of F‐ERK were 2 and 6 ng/mL and those of P‐F‐ERK were 4 and 12 ng/mL. The correlation coefficients obtained from two standard curves were approximately 0.99. The reproducibility and accuracy of the method for F‐ERK ranged 1.5–4.7 and 86–109%, respectively, and those for P‐F‐ERK were 1.6–6.1 and 93–109%, respectively. The activity of PKCδ was studied in vitro using the human gastric cancer cell line MKN‐1. The use of PKCδ inhibitor candidates, including G?6983, bisindolylmaleimide II, staurosporine, and rottlerin in the assay resulted in IC50 values of 50 nM, 15 nM, 795 nM, and 4 μM, respectively. Comparison of our assay with a commercial PKC kit revealed that our assay is more adaptable to differing enzyme isoforms. This method has potential for high throughput screening for kinase inhibitors as part of a drug discovery program. 相似文献
95.
Byung Woo Yoo Min Kyung Lee Cheol Min Yoon 《Phosphorus, sulfur, and silicon and the related elements》2016,191(6):807-810
The TaCl5/In system was found to be a new reagent for reducing a wide range of structurally diverse sulfoxides to the corresponding sulfides with high yields under mild conditions. This protocol is chemoselective and tolerates several functional groups, such as Br, Cl, OCH3, CHO, and CH = CH2. 相似文献
96.
Summary This paper is a further contribution to our work on the Weissenberg effect. One goal of our work is to show how the free-surface deformations on a viscoelastic fluid which is sheared between two concentric rotating cylinders can be used to determine rheological data about the fluid. In this paper we report the results of an experimental program in which free surface shapes have been measured on three viscoelastic fluids undergoing shearing in an apparatus consisting of a stationary outer cylinder and four interchangeable rotating inner cylinders. Experimentally-measured profiles are compared with profiles predicted from second-order theory, and found to be in excellent agreement for STP and TLA-227 in the range of applicability of the second-order theory, but there is some disagreement in the results for a 2% solution of polyacrylamide in 49% water –49% glycerin.
With 12 figures and 1 table 相似文献
Zusammenfassung Diese Arbeit stellt einen weiteren Beitrag zu unseren Untersuchungen über den Weissenberg-Effekt dar. Ein Ziel dieser Untersuchungen ist es zu zeigen, wie die Deformation der freien Oberfläche einer zwischen zwei konzentrischen Zylindern gescherten viskoelastischen Flüssigkeit dazu verwendet werden kann, rheologische Kenngrößen der Flüssigkeit zu gewinnen. In dieser Arbeit wird über die Ergebnisse einer experimentellen Untersuchung berichtet, in der die Gestalt der freien Oberfläche bei drei viskoelastischen Flüssigkeiten gemessen wurde, die in einem Apparat geschert wurden, der aus einem stationären Außenzylinder und vier auswechselbaren rotierenden Innenzylindern bestand. Die experimentell bestimmten Profile werden mit solchen verglichen, die aufgrund einer Theorie zweiter Ordnung vorausgesagt worden sind. Man findet im Bereich der Anwendbarkeit dieser Theorie für STP und TLA-227 eine ausgezeichnete Übereinstimmung, wohingegen die Ergebnisse bei einer 2%igen Polyacrylamid-Lösung in einer 1:1-Wasser-Glycerin-Mischung gewisse Diskrepanzen aufweisen.
With 12 figures and 1 table 相似文献
97.
3,4-Dihydro-2H-pyrrole derivatives were synthesized by the 1,3-dipolar cycloaddition reaction of nitrile ylides with acrylamides. Acrylamide substitution patterns and benzimidoyl chloride equilibration were investigated. 相似文献
98.
99.
Dong Chan Kim Kyung Ho Yoo Kye Jung Shin Sang Woo Park Dong Jin Kim 《Journal of heterocyclic chemistry》1997,34(1):57-63
The pKa′s of several thiazole heterocyclic compounds have been determined and represent products with significantly high values. Because of their high basicities, sometimes these compounds were able to act as not only nucleophiles but also strong organic bases. 4-Substituted-3-methyl-2-methylimino-Δ4-thiazolines 5a-d reacted with ethyl bromoacetate in refluxing benzene, giving the corresponding N-alkylated salts 8a-d , while the products obtained from the reaction with 2-bromoacetophenone in the presence of base were pyrrolothiazines 10b-d. 相似文献
100.
Current (I)–voltage (V) characteristic under oxygen potential gradients was experimentally examined on single crystal BaTiO3−δ in its mixed ion/electron/hole regime at 1000 °C. The variation of I vs. V appears similar to that of an n/p junction, but with the limiting slope (dI/dV) approaching the maximum and minimum possible equilibrium conductances in the given oxygen potential gradient as increasing forward and reverse bias, respectively. This characteristic has been precisely traced theoretically by using the partial ionic and electronic conductivities of BaTiO3−δ as measured against uniform oxygen chemical potential in equilibrium state. The nonlinear characteristic is attributed to the redistribution of oxygen chemical potential that is caused by a non-vanishing gradient of the ionic transference number of the oxide under the given oxygen potential gradient. It is demonstrated that the bulk transport properties of a mixed conductor may be tailored by terminal voltage in a chemical potential gradient. 相似文献